The Effect of Sn(IV) Chlorin e6 on HepG2 Cancer Cell Lines

Abstract

This research investigated the effect of Sn(IV) chlorin e6 dichloride trisodium salt photosensitizer on the viability of HepG2 cancer cell lines in vitro. The effect of light on the viability of cells without the photosensitiser and the toxicity of the photosensitiser in the absence of light were examined in this research. No toxic effects with the absence of light were found and no photodamge effect on the cells without the presence of photosensitiser. The effect of different concentrations of the photosensitiser with fixed light dose of 70 J/cm2 on the viability of HepG2 cancer cells were performed. Then, two concentrations 25 and 30 μg/ml were investigated at different light doses 60-100 J/cm2. The effect of the photosensitiser on the viability of HepG2 at different light doses and different concentrations was found to have low viability over concentrations of 25 μg/ml. This could be due to that the photosensitiser reached a saturated status after this concentration.
Key words: PDT; Sn(IV) chlorin e6; liver cancer; phototoxicity; viability assay